Radiation protection concept and philosophy is in news over the past several decades. The inadvertent exposure of human being from various sources including the planned radiotherapy for the treatment of cancer causes ionization of molecules setting of potentially damaging reactions via free radical production affecting the homeostasis of cells and causing deleterious effects. Despite extensive screening of several synthetic compounds for radioprotective activity, no single compounds has been emerged as a good radioprotector which can be used with minimum side effects. Single plant extracts and purified phytochemicals are studied for their radioprotective property. We have utilized a polyherbal formulation PRA-5 ( 80 % hydroalchoholic extract of leaves of W. somnifera, stem of T. cardifollia, pulp of T. belerica, tuber of C.longa and leaves of O.sanctum in equal proportion ) based on the concept of synergetic action of polychemical/s on individual and mixture of extracts exerting their effect on the principle of polypharmacy to evaluate the radioprotective property in juice exposed to gamma radiation. The radioprotective effects were observed in both in vitro and in vivo. For in vivo experiments of antioxidant activity was noted using various models, plasmid DNA and lymphocyte comet assay. Radioprotective effect was confirmed by using various hematological, immunological histological and biochemical parameters in mice exposed to radiations. Additionally antimicrobial and plasmid curing action of multiple drug resistance bacteria was also noted. The results were subjected to statistical analysis proving significant radioprotective action of PRA-5.
