An improved centrifuge system having a single means for both feeding and collecting liquid streams aseptically from rotating components is provided. Also methods and apparatus for centrifugal separation of cells from cell culture media of large cell culture batches by processing a large volume within a few hours, using pre-sterilized, single-use fluid path components. The apparatus uses a sealing approach that improves reliability while avoiding air contamination as well as shedding from mechanical seals. The risk of process liquid leaks is minimized.
