ZHANG Pengjun,LIU Guangfu,FU Xianshu,XU Yipeng,YANG Qianqian,SHENTU Xuping,YU Xiaoping,SUN Kai
申请号：
LU101793
公开号：
LU101793B1
申请日:
2020.05.15
申请国别(地区):
LU
年份:
2020
代理人:
摘要：
Provided is a single-chain antibody TRsAb2 against ToRSV, having an amino acid sequence shown in SEQ ID NO.l, and consisting of a 15 aa histidine tag region, a 184 aa ToRSV heavy chain variable region, a 15 aa linker peptide, and a reverse-linked 102 aa ToRSV light chain variable region, connected in sequence, with a length of 316 aa residues. The sequences of the 184 aa heavy chain variable region and the 102 aa light chain variable region are derived from the sequence of a monoclonal antibody produced by the hybridoma cell line CGMCCl 8320. The TRsAb2 gene is obtained by a whole gene synthesis method, and the codon composition is optimized. The single-chain antibody TRsAb2 of the invention has the characteristics of selectively identifying the peptide AFGKGVEEIEQTST in the ToRSV coat protein, and has a positive response to natural ToRSV particles.
