GURNEY AUSTIN L,BOND CHRISTOPHER J,LAZETIC ALEXANDRA L. L
申请号：
NZ60174311
公开号：
NZ601743A
申请日:
2011.02.11
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Disclosed is a polypeptide comprising: (i) an extracellular portion that comprises an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains and does not comprise an immunoglobulin heavy chain variable region and (ii) a non-immunoglobulin transmembrane portion. The polypeptide comprises a sequence as defined in the complete specification. Also disclosed is a method of identifying a cell that produces a heterodimeric molecule of interest comprising: (a) transfecting a cell that expresses a first polypeptide with a polynucleotide that encodes at least one second polypeptide with the first polypeptide comprising (i) an extracellular portion that comprises an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains and does not comprise an immunoglobulin heavy chain variable region and (ii) a nonimmunoglobulin transmembrane portion with the second polypeptide comprises an immunoglobulin heavy chain and wherein a heterodimeric molecule comprising the first polypeptide and the second polypeptide is expressed on the surface of the cell (b) contacting the cell with a detection molecule, wherein the detection molecule binds the heterodimeric molecule and (c) identifying the cell bound by the detection molecule with the method not being performed within a human being.
