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Anordning og fremgangsmåde til transfektion med stor volumen
专利权人:
Lonza Cologne GmbH
发明人:
Altrogge, Ludger,Gleissner, Timo,Heinze, Andreas,Hermsmeier, Sven
申请号:
DK20140191272T
公开号:
DK2940121(T3)
申请日:
2014.10.31
申请国别(地区):
丹麦
年份:
2018
代理人:
摘要:
The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space 40 for holding the suspension, the internal space 40 comprising at least two segments 41, 42, wherein each segment 41, 42 comprises at least one electrode 43, 44 and wherein neighboring electrodes 43, 44 are separated from each other by at least one gap 47 which is at least partially filled with an insulating material 46, and wherein the edges of the electrodes 43, 44 facing each other within the internal space 40 are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode 43, 44 while the electrodes 43, 44, 45 or electrode segments next and/or opposite to the active electrode 43, 44 are set to ground potential. Setting neighboring electrodes
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