Callen, Walter,Richardson, Toby,Frey, Gerhard,Short, Jay M.,Kerovuo, Janne S.,Mathur, Eric J.,Slupska, Matgorzata,Gray, Kevin
申请号：
ES10184415
公开号：
ES2604329T3
申请日:
2002.02.21
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
An isolated, synthetic or recombinant nucleic acid comprising: (A) (i) a sequence encoding a polypeptide having alpha amylase activity, wherein said sequence has at least 95%, 96%, 97%, 98% , 99% or 100% sequence identity with the sequence of SEQ ID NO: 77 or enzymatically active fragments thereof, in which the fragment has alpha amylase activity, or (ii) the sequence of (i), wherein the sequence identity is determined by analysis with a sequence comparison algorithm, or determined by visual inspection, or (iii) the sequence of (ii), in which the sequence comparison algorithm comprises a 3.0 algorithm t78 of FASTA version with default parameters； (B) a nucleic acid comprising (i) a sequence encoding a polypeptide having alpha amylase activity, in which the amino acid sequence of the polypeptide has at least 97%, 98%, 99% or 100% identity sequence with the amino acid sequence of SEQ ID NO: 78 or enzymatically active fragments thereof, in which the fragment has alpha amylase activity, or (ii) the sequence of (i), in which the sequence identity by analysis with a sequence comparison algorithm, or is determined by visual inspection, or (iii) the sequence of (ii), in which the sequence comparison algorithm comprises a 3.Ot78 algorithm of FASTA version with default parameters； (C) a nucleic acid comprising a sequence of any of (A) to (B) encoding a polypeptide that lacks a signal sequence； (D) a nucleic acid comprising: (i) a sequence of any of (A) to (C) encoding a polypeptide further comprising a heterologous nucleic acid sequence； (ii) the sequence of (i), in which the heterologous nucleic acid sequence encodes a signal sequence； (iii) the sequence of (ii), wherein the heterologous signal sequence comprises an amylase signal sequence； or (E) a nucleic acid sequence completely complementary to any nucleic acid sequence from (A) to (D).Un ácido nucleico aislado, sintético o recombinante que comprende: (A) (i) una secuencia que codifica un polipéptido que tiene actividad
