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Purification de polysaccharides capsulaires bactériens
专利权人:
GlaxoSmithKline Biologicals SA
发明人:
申请号:
EP10179797.5
公开号:
EP2277536B1
申请日:
2002.06.20
申请国别(地区):
EP
年份:
2018
代理人:
摘要:
Precipitated bacterial capsular polysaccharides can be efficiently re-solubilised using alcohols as solvents. The invention provides a process for purifying a bacterial capsular polysaccharide, comprising the steps of (a) precipitation of said polysaccharide, followed by (b) solubilisation of the precipitated polysaccharide using ethanol. CTAB can be used for step (a). The material obtained, preferably following hydrolysis and sizing, can be conjugated to a carrier protein and formulated as a vaccine. Also, in vaccines comprising saccharides from both serogroups A and C, the invention provides that the ratio (w/w) of MenA saccharide : MenC saccharide is >;1.
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