SMITH, Jonathan F.,Kamrud, Kurt,Dryga, Sergey,Alterson, Harold,Rayner, Jon,Alterson, Kim,Maughan, Maureen F.
申请号：
DK10011537
公开号：
DK2290054T3
申请日:
2003.12.12
申请国别(地区):
DK
年份:
2017
代理人:
摘要：
Provided herein are methods for producing alphavirus replicon particles in high yield； replicon RNAs are electroporated into permissive cells, where the cells are at a relatively high density, together with at least one helper nucleic acid providing the necessary functions for packaging. After a growth period in appropriate medium, alphavirus replicon particles are harvested from the surfaces of the cells in which they were produced using a salt wash in which the salt concentration is from about 0.2 to about 5 M sodium chloride, calcium chloride, magnesium chloride, potassium chloride, ammonium acetate, ammonium bicarbonate, among others. After dilution, if necessary, the particles can be purified by a suitable chromatographic technique.
