Disclosed are a type of tridentate linkers and use thereof. The tridentate linkers can be used to prepare an antibody drug conjugates as represented by formula I, wherein, L is an antibody, antibody fragment or protein； T is a tridentate linker part； A is a cleavable linker group or a noncleavable linker part； D is a drug part； n is an integer of 0-8. The structure of the tridentate linker part is as represented by formula II, wherein, W is substituted aryl, heteroaryl, linear alkyl, cycloalkyl, heterocycloalkyl, or any combination thereof. L -(T-A-D)n I
