The invention relates to immunostimulatory oligonucleotides and methods of using immunostimulatory oligonucleotides to induce an antigen-specific immune response. The invention further relates to a vaccine that comprises an immunostimulatory oligonucleotide and an antigen, and comprises a pharmaceutically acceptable carrier. The immunostimulatory oligonucleotides of the invention, in some embodiments, include one or more modified linkage(s).本發明係關於免疫刺激性寡核苷酸，及使用免疫刺激性寡核苷酸誘導抗原特異性免疫反應之方法。本發明另外關於包含免疫刺激性寡核苷酸及抗原及包含醫藥上可接受之載劑之疫苗。在一些實施例中，本發明免疫刺激性寡核苷酸包括一或多個經修飾之鍵聯。
