The present invention relates to an automated blood vessel preparation apparatus, which comprises an inclined stage in the inside, and a sort of blood vessel collection piles of different types are loaded horizontally on the inclined stage irrespective of the direction of the blood collection vessel, At least one blood collection tube feeding means for dropping the blood vessels one by one from the conveyance passage; Conveying means for moving the blood vessel to the label attaching means by driving the conveyance belt while being separated from the conveyance passage by the blood collecting tube feeding means; A stopper which is moved by the feeding means and stops the blood vessel while reaching the label attaching means; A sensing unit provided in the blood collecting tube feeding means for sensing the presence or absence of a blood vessel waiting to fall into the transfer path and a direction in which the blood vessel is placed; And a control unit that drives the corresponding blood supply unit according to the type and number of blood vessels to be prepared and stops the blood collection vessel according to the type and direction of the blood collection vessel when the presence or direction of the blood collection vessel is detected by the detection unit Wherein the lower end of the inclined stage is spaced apart from the inner side wall of the blood vessel supplying means by a dropping space so that the blood collection vessels can descend one by one, Providing a preparation device. According to the present invention, the collection vessel is stacked on the inclined stage in a horizontal state regardless of the direction of the blood collection vessel, and the blood vessel is supplied to the blood vessel supply means for receiving and supplying the blood vessel by dividing the blood vessels according to the type thereof. By moving the blood vessels one by one from the blood vessels to the transfer path and then moving them to the labeling means using the hori