GOLETZ, STEFFEN,DANIELCZYK, ANTJE,BAUMEISTER, HANS,STAHN, RENATE,LOFFLER, ANJA,STOCKL, LARS
申请号：
NZ57597407
公开号：
NZ575974A
申请日:
2007.09.10
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed is an isolated immortalized human blood cell selected from the group consisting of NMH9D8 [DSM ACC 2806], NM-H9D8-E6 [DSM ACC 2807], NM-H9D8-E6Q12 [DSM ACC 2856] or a cell or cell line derived therefrom, which is capable of producing a protein molecule composition having the following glycosylation characteristics: (i) it comprises no detectable NeuGc； (ii) it comprises no detectable terminal Galalpha1-3Gal； (iii) it comprises at least 2% carbohydrate structures of the total carbohydrate units or of at least one particular carbohydrate chain at a particular glycosylation site of a protein molecule of the protein molecules in said protein molecule composition which contains bisecting GlcNAc； (iv) it comprises alpha2-6 linked NeuNAc； (v) it has an increased sialylation degree with an amount of NeuNAc on the total carbohydrate structures or on the carbohydrate structures at one particular glycosylation site of the antibody molecule of said antibody molecules in said antibody molecule composition which is at least 15% higher compared to the same amount of antibody molecules in at least one antibody molecule composition of the same antibody molecule isolated from CHOdhfr [ATCC No. CRL-9096] when expressed therein. Further disclosed is a method for producing a protein molecule composition, comprising (a) introducing in a host cell which is an immortalized human blood cell at least one nucleic acid encoding at least a part of said protein； and (b) culturing said host cell under conditions which permit the production of said protein molecule composition； and (c) isolating said protein molecule composition； Wherein the host cell is a cell as defined above.
