The Board of Trustees of the Leland Stanford Junior University
发明人:
Yang Samuel,Deisseroth Karl A.,Allen William E.,Kauvar Isaac V.,Andalman Aaron S.
申请号：
US201615735550
公开号：
US2018177401(A1)
申请日:
2016.06.13
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
A method for measuring the activity of one or more excitable cells, such as neurons, in a target tissue is provided. The present method may include measuring the activity of individual, selected excitable cells by projecting one or more three dimensional (3D) multi-focal laser light patterns into a target tissue containing excitable cells adapted to emit cellular electrical activity—sensitive fluorescence, to generate a multiplexed 2D diffraction pattern of fluorescence emitted by the neurons, and resolving the multiplexed 2D diffraction pattern. Also provided herein is a system configured to perform the present method, the system including a microscope configured to project one or more 3D multi-focal laser light patterns into a target tissue using a spatial light modulator and a mirror galvanometer, and a microlens array and an image detector to record individual and multiplexed 2D diffraction patterns of light emitted from the target tissue.
