The presently claimed invention is related to a method for vegetative propagation of poplar trees (Populus sp.), from prepared leaf cuttings, wherein the method comprises the steps of: a) providing cuts of leaf cuttings from a donor tree, b) immersing the prepared leaf cutting into a combination of growth regulators, c) planting the prepared leaf cutting into a solid substrate of organic and/or inorganic origin, d) inducing sprouting and rooting of the prepared leaf cutting, by means of controlled ambient temperature, humidity and illumination, wherein steps a) through d) always occur outside an in vitro environment and embodiments thereof.
