PROBLEM TO BE SOLVED: To provide a method for highly efficiently producing a retinal pigment epithelial cell.SOLUTION: There is provided a method for producing a retinal pigment epithelial cell, comprising following steps of (1) to (4), wherein (1) a first step of subjecting a pluripotent stem cell to floating culture in a serum-free culture medium containing a Wnt signaling pathway inhibitor to form an aggregate of the pluripotent stem cells, (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free culture medium containing a basement membrane preparation, (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing culture medium, and (4) a fourth step of subjecting the aggregate cultured in the third step to floating culture in a serum-free culture medium or a serum-containing culture medium each containing a substance capable of acting on the Wnt signaling pathway (wherein the serum-free culture medium and the serum-containing culture medium do not contain any substance acting on the Sonic hedgehog signaling pathway).【課題】高効率な網膜色素上皮細胞の製造方法を提供する。【解決手段】下記（１）～（４）の工程を含むことを特徴とする網膜色素上皮細胞の製造方法。（１）多能性幹細胞を、Wntシグナル経路阻害物質を含む無血清培地中で浮遊培養することにより多能性幹細胞の凝集体を形成させる第一工程（２）第一工程で形成された凝集体を、基底膜標品を含む無血清培地中で浮遊培養する第二工程（３）第二工程で培養された凝集体を、血清培地中で浮遊培養する第三工程、及び（４）第三工程で培養された凝集体を、Wntシグナル経路作用物質を含む無血清培地又は血清培地（但し、前記無血清培地及び血清培地は、ソニック・ヘッジホッグシグナル経路作用物を含まない）中で浮遊培養する第四工程【選択図】なし
