The application of the procedure through a natural orifice of the present invention includes the steps of: inserting a flexible endoscope through the natural orifice to the alimentary tract incising the close-contact portion of the alimentary tract and the gall bladder under a guidance of a device for identifying the positional relationship from the alimentary tract side and forming an puncture extracting stones in the gall bladder from the body and removing the endoscope after completing the procedure.