FLORIAN MARTIN,GREGOR NICOLAS BINDLER,MICHEL PHILIPPE BLANC,NICOLAS BAKAHER,SIMON GOEPFERT
申请号:
BR112014004827
公开号:
BR112014004827A8
申请日:
2012.08.31
申请国别(地区):
BR
年份:
2018
代理人:
摘要:
abstract the present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to seq id no: 1 or seq id no: 10 or seq id no: 12 or seq id no: 14; or (ii) the polypeptide encoded by said polynucleotide (s); or (iii) a polypeptide having at least 60% sequence identity to seq id no: 2 or seq id no: 11 or seq id no: 13 or seq id no: 15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence (s), optionally wherein said construct, vector or expression vector additionally comprising a promoter comprising, consisting or consisting essentially of the sequence set forth in seq id: 8 or a variant thereof with at least about 60% identity thereto or a trichome promoter. -------------------------------------------------- -------------------------- Translation of abstract patent summary: "isopropylmalate synthesis of nicotiana ta-bacum and methods and uses thereof". The present invention relates to a mutant transgenic or non-naturally occurring plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropyl malate synthase and having at least 60% of string identity for seq id no: 1 or seq id no: 10 or seq id no: 12 or seq id no: 14; or (ii) a polypeptide encoded by said polynucleotide (s); or (iii) a polypeptide having at least 60% sequence identity for sequence id: 2 or sequence id: 11 or sequence id: 13 or sequence id: 15; or (iv) an expression construct, vector or vector comprising said polynucleotide sequence (s), optionally wherein said expression construct, vector or vector further comprises a promoter comprising, consisting of or consisting essentially of the sequence shown below. id no: 8 or a variant thereof of at least about 60% identity to it or a trichome