[MEANS FOR SOLVING PROBLEMS] The present invention provides a culture medium composition for mesenchymal stem cells for cancer treatment that suppresses the proliferation of cancer cells while maintaining differentiation ability and activity, and a mesenchymal stem cell for cancer treatment using the composition. More particularly, the present invention relates to a medium composition for producing mesenchymal stem cells containing vitamin C and aspirin and having an improved ability to suppress cancer cell growth, and a method for producing mesenchymal stem cells for cancer treatment using the composition.【課題】【解決手段】本発明は分化能と活性を維持しながら癌細胞の増殖を抑制する癌治療用間葉幹細胞の培養培地組成物及び前記組成物を用いた癌治療用間葉幹細胞の製造方法に係り、より詳しくはビタミンCとアスピリンを含む改善した癌細胞増殖抑制能を有する間葉幹細胞製造用培地組成物及び前記組成物を用いた癌治療用間葉幹細胞の製造方法に関する。