The method for regulating a transit of wild animals along a crossing pathway of a public road, the pathway being habitually used by wild animals and meeting the public road (1) at a crossing (3), comprises following steps: a) monitoring a portion (20) of the pathway (2) in proximity of the crossing (3), for detecting presence of a wild animal (4) in proximity of the crossing (3); b) monitoring a portion (10) of the public road (1) in proximity of the crossing (3), for detecting a presence of at least a vehicle (5) in proximity of the crossing (3) and a velocity (V) thereof; c) allowing transit of the wild animal (4) detected on the crossing (3) if a vehicle (5) presence is not detected in proximity of the crossing (3) or alternatively if a presence of a vehicle (5) is detected in proximity of the crossing (3) travelling at a velocity (V) that is lower than a predetermined limit value (V*); d) prohibiting transit of the wild animal (4) detected in proximity of the crossing (3) if a presence is detected of a vehicle (5) in proximity of the crossing (3) having a velocity (V) of higher than the predetermined limit value (V*).