SOUTH DAKOTA BOARD OF REGENTS;CAMBRIDGE ENTERPRISE LIMITED;ACADEMISCH ZIEKENHUIS LEIDEN;UNIVERSITY COLLEGE CORK
发明人:
Ying Fang,Eric John Snijder,Andrew E. Firth,John F. Atkins,Emma Elisabeth Treffers,Ali Tas,Yanhua Li
申请号：
US14413385
公开号：
US09623103B2
申请日:
2013.07.18
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The invention provides the discovery and characterization of a novel arterivirus protein (nsp2TF), whose expression is dependent on −2 ribosomal frameshifting at a site located in the nsp2 coding region. The coding region for the unique TF domain of nsp2TF overlaps the part of ORF1a that encodes the transmembrane region of nsp2 in arteriviruses, including PRRSV, LDV and SHFV. Mutations affecting the expression of nsp2TF impair PRRSV replication and result in a smaller plaque phenotype. Provided herein are arteriviruses that display reduced translation of nsp2TF and/or altered translation of one or more downstream products, arteriviruses in which nsp2TF function is reduced and/or absent, and vaccines comprising said arteriviruses. Also provided herein are diagnostic methods, methods for identifying compounds that inhibit −2 frameshifting, and gene expression tools for eukaryotic systems utilizing −2 frameshifting.
