A use of butylidenephthalide (BP) in the manufacture of a preparation is provided, wherein the preparation is a medicament, a food, or a food additive for preventing, mitigating and/or inhibiting the growth, migration, invasion and/or metastasis of cancer stem cells (CSCs).一種使用亞丁基苯酞(butylidenephthalide,BP)於製造一製劑的用途,其中該製劑係一藥劑、一食品、或一食品添加劑,用於預防、減緩及/或抑制癌症幹細胞(cancer stem cells,CSCs)之生長、遷移(migration)、侵襲(invasion)及/或轉移(metastasis)。