The present invention relates to a method for isolating a large amount of damulin A and damulin B which are active components activating AMPL useful for treating metabolic disease including such as obesity, diabetes, and hyperlipidemia from an extract of Gynostemma pentaphyllum. Through the refining and manufacturing process of the present invention, a large amount of the damulin A and the damulin B which are active components activating AMPK can be manufactured and isolated.본 발명은 돌외 추출물로부터 비만, 당뇨, 고지혈증 등을 포함하는 대사질환의 치료에 유용한 AMPK 활성화 유효 성분인 다물린 A 및 다물린 B를 대량으로 분리하는 분리방법에 관한 것이다.