MAMBRINI Dzhovanni (IT),МАМБРИНИ Джованни (IT),BENATTI Luka (IT),БЕНАТТИ Лука (IT),KAPOGROSSI Dzhovanni (IT),КАПОГРОССИ Джованни (IT),MANDOLINI Marko (IT),МАНДОЛИНИ Марко (IT)
申请号：
RU2015152801
公开号：
RU0002670070C2
申请日:
2014.05.09
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: invention relates to cellular biology and biotechnology, in particular to a method for producing erythrocytes, to erythrocytes and a pharmaceutical composition. Method includes swelling erythrocytes using a first hypotonic solution, which results in erythrocytes having osmolality of 250–200 mOsm/kg. Method includes further swelling of the erythrocytes without reaching lysis, using a second hypotonic solution which is more hypotonic than the first hypotonic solution and which results in the erythrocytes having osmolality of 200–170 mOsm/kg. Further, the erythrocytes are concentrated and the concentrated erythrocytes are brought into contact with a lysing solution containing one or more substances of pharmaceutical interest, which results in the erythrocytes having osmolality of 150–110 mOsm/kg. Further, a sealing solution is added to obtain an erythrocyte population, which is characterised in that the percentage of phosphatidylserine formed by the erythrocytes, which is measured in annexin V assay, is less than 10 % and the amount of lactate formed for every 106 erythrocytes is 0.100 nmol/h. Resulting erythrocytes can be used as part of a pharmaceutical composition for use in therapeutic treatment.EFFECT: invention makes it possible to obtain erythrocytes loaded with one or more substances of pharmaceutical interest, avoiding mechanical stress and simplifying the sealing step.18 cl, 4 dwg, 8 tbl, 9 exИзобретение относится к области клеточной биологии и биотехнологии, в частности к способу получения эритроцитов, эритроцитам и фармацевтической композиции. Способ включает набухание эритроцитов с использованием первого гипотонического раствора, который приводит эритроциты к осмоляльности 250-200 мОсм/кг. Далее осуществляют дополнительное набухание эритроцитов, не достигая лизиса, с использованием второго гипотонического раствора, который более гипотоничен, чем первый гипотонический раствор, и который приводит эритроциты к осмоляльности 200
