PROBLEM TO BE SOLVED: To provide an adsorbent having a high antibody adsorption amount, which is obtained by immobilizing an affinity ligand capable of specifically binding to an antibody on an insoluble carrier, and which can also be used for the purpose of fractionating antibodies. thing. SOLUTION: An antibody adsorbent obtained by immobilizing an Fc-binding protein on porous hydrophilic polymer particles having a particle size of 7 μm or more and 25 μm or less, and an equilibration buffer is passed through a column packed with the adsorbent. It includes a step of equilibrating a column, a step of adding a solution containing an antibody to the equilibrated column to adsorb the antibody to the adsorbent, and a step of eluting the antibody adsorbed to the adsorbent with an eluent. The above-mentioned problems are solved by an antibody separation method. [Selection diagram]【課題】 抗体と特異的に結合可能なアフィニティリガンドを不溶性担体に固定化して得られる抗体吸着剤であって、抗体の分取目的にも利用可能な、抗体吸着量の高い吸着剤を提供すること。【解決手段】 Ｆｃ結合性タンパク質を粒子径７μｍ以上２５μｍ以下の多孔質親水性ポリマー粒子に固定化して得られる抗体吸着剤、ならびに前記吸着剤を充填したカラムに平衡化緩衝液を通液してカラムを平衡化する工程と前記平衡化したカラムに抗体を含む溶液を添加して抗体を前記吸着剤に吸着させる工程と前記吸着剤に吸着した抗体を溶出液を用いて溶出させる工程とを含む抗体の分離法により、前記課題を解決する。【選択図】 図２
