A method of using a biological sampling device to obtain a biological sample. The device comprises a sheath, an applicator, a collector, and a handle. The method includes inserting a first applicator end into an organism with the device being in a pre-sampling configuration； transitioning the device from the pre-sampling configuration to a sampling configuration； with the device in the sampling configuration, via the handle, moving the collector against biological material within the organism to collect the biological material； transitioning the device from the sampling configuration to a post-sampling configuration； and with the device in the post-sampling configuration, withdrawing the device from within the organism.
