The present invention provides a method and device for concentration and detection of a target analyte in a solution using a pre-coated porous material embedded with ATPS components. In one embodiment, the water soluble nanoparticles include, but are not limited to, silicon dioxide, iron oxide, titanium dioxide, silver oxide and any combinations thereof. In one embodiment, the present invention provides a device comprising ATPS as a concentration module linked with a lateral flow immunoassay (LFA) component as a detection module. In one embodiment, as the sample wicks up the device and ATPS components undergo phase separation, the analytes are concentrated substantially in the leading front, the concentrated analytes are then detected by the LFA module generating visual test results. In one embodiment, the present method and device are used to detectStreptococcus mutansand thereby dental caries.本發明提供了一種用嵌入ATPS 組分的預塗多孔材料來濃縮和檢測溶液中的靶標分析物的方法和設備。在一個實施例中,水溶性納米粒子包含,但不限於,二氧化矽,氧化鐵,二氧化鈦,氧化銀及其任何組合。在一個實施例中,本發明提供了一種設備,該設備包含 ATPS 組分作為濃縮模塊與作為檢測模塊的橫向流動免疫分析法 (LFA) 相連接。在一個實施例中,當樣品在毛細管作用下被汲取進入裝置和ATPS組分經過相分離,其分析物在流體流動的前沿位置濃縮。然後,已濃縮的分析物經過檢測模塊檢測得到可見測試結果。在一個實施例中,本方法和設備用於檢測導致齲齒的變異鏈球菌。
