To provide anti-BRDU antibodies and methods of use.SOLUTION: Disclosed herein is a humanized anti-BRDU antibody comprising HVR-H1, HVR-H2, HVR-H3, HVR-L1, HVR-L2 and HVR-L3 each comprising a specific amino acid sequence； where the antibody has: 1) P at position 30 of the heavy chain variable domain, 2) F at position 58 of the heavy chain variable domain； 3) T at position 108 of the heavy chain variable domain； 4) K at position 49 of the light chain variable domain； and 5) L at position 98 of the light chain variable domain (numbering according to Kabat). Also disclosed is a complex comprising the humanized anti-BRDU antibody and a nucleic acid comprising BRDU.SELECTED DRAWING: None【課題】抗BRDU抗体およびその使用方法を提供する。【解決手段】特定のアミノ酸配列を含むHVR-H1、HVR-H2、HVR-H3、HVR-L1、HVR-L2、およびHVR-L3を含み、１）重鎖可変ドメインの30位にP、および２）重鎖可変ドメインの58位にF、および３）重鎖可変ドメインの108位にT、および４）軽鎖可変ドメインの49位にK、および５）軽鎖可変ドメインの98位にL（位置はいずれもKabatに従う）を含む、ヒト化抗BRDU抗体。およびヒト化抗BRDU抗体と、BRDUを含む核酸とを含む、複合体。【選択図】なし
