The present invention relates generally to compositions for wound healing. More specifically, the present invention provides human skin equivalents engineered to express exogenous proteinase inhibitor polypeptides (e.g., TIMP-1 polypeptides) and compositions and methods for making human skin equivalents engineered to express exogenous polypeptides. In addition, the present invention provides methods for treatment of wounds with human skin equivalents engineered to express exogenous polypeptides.
