A manual venous catheter assembly (10) includes a catheter fixing barrel (20), a catheter (30) provided at the catheter fixing barrel (20), a puncture needle fixing barrel (40) movably sleeved onto the catheter barrel (20), a puncture needle (50) fixed to the puncture needle fixing barrel (40), and a positioning ring base (60) fixed to the puncture needle fixing barrel (40) and surrounding the puncture needle (50). When the puncture needle fixing barrel (40) moves to an extracted position (P2), the puncture needle (50) is pulled to depart from the catheter (30), such that the puncture needle (50) is located in the catheter fixing barrel (20) to avoid exposure, and the positioning ring base (60) is abutted and engaged with the catheter fixing barrel (20) to prevent separation between the catheter fixing barrel (20) and the puncture needle fixing barrel (40), thus achieving the effects of simplifying the structure and facilitating assembly.
