Julio César Aguilar Rubido,Yadira Lobaina Mato,Dunia Hernández Inguanzo,Heidy Trujillo Pérez,Freya de los Milagros Freyre Almeida,Verena Lucila Muzio González,Eduardo Pénton Arias,Sonia Gonzalez Blanc
申请号：
US13498842
公开号：
US20120308615A1
申请日:
2010.09.28
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The present invention is related with the field of the therapeutic immunization, specifically with the employment of a new formulation of antigens of the Hepatitis B Virus (HBV) for the cellular stimulation. The formulation is formed by the surface antigens (HBsAg) precipitated in suspension and the nucleocapsid (HBcAg) of the HBV. The formulation contains these antigens like suspended particles of sizes less than 500 nm and higher than 500 nm, in a mixture where the proportion among the particles of the mentioned sizes is among 50%-50% and 80%-20%, respectively. The selection of a range of sizes of particles allows that the levels of stimulation of several cellular types are maximized. Additionally, a cellular stimulation method is described with this formulation, and the later passive immunization of patients with chronic Hepatitis B, based on the maximum stimulation in vivo or in vitro using heterologous or autologous cells (dendritic cells, B cells and macrophages). The cells stimulated with this formulation are transferred to patients chronically infected with the HBV.
