The present invention relates to vaccines for waterfowl immunization based on live recombinant Mareks disease virus (hereinafter referred to as rMDV), and more specifically recombinant herpes virus of turkeys (hereinafter referred to as rHVT virus), which incorporates at least one nucleotide sequence encoding and expressing an antigenic peptide of a pathogenic agent of waterfowl. Methods of immunization are also provided.