METHOD FOR SELECTING HAPLOID OR DUPLICATE DENGULATED DENGLYPED PLANT HYDROPLE PLANT, PLANT, CLONES, POLLES OR OVERS OF HYBRID TREES OR SEEDS, HARVESTING AND EXTRACTED PRODUCTS AND METHOD FOR OBTAINING OIL.
STEPHEN PETER CONNOR NELSON,JAMES MARTIN DUNWELL,MICHAEL JAMES WILKINSON,PETER DOUGLAS SAVARIA CALIGARI
申请号：
BRPI0809020
公开号：
BRPI0809020A2
申请日:
2008.03.19
申请国别(地区):
BR
年份:
2014
代理人:
摘要：
The present invention relates to haploid oil palm plants and homozygous doubled haploid oil palm plants. The invention also relates to methods for producing and selecting haploid and doubled haploid plants. More particularly, but not exclusively, the method may be used for selecting haploid and doubled haploid oil palm plants. Haploid and doubled haploid plants are selected by a large-scale screening based on a combination of the phenotype with the use of molecular methods combined with flow cytometry techniques to identify haploid and doubled haploid plants. More particularly, a method for selecting haploid and doubled haploid plants is described comprising: (a) germinating seeds； (b) selecting seedlings with atypical phenotype； (c) assessing heterozygosity using markers； (d) isolating cells from the seedlings and determining the DNA content of the cells； and (e) isolating and purifying the DNA and using defined molecular markers to characterise the genotype of the plant. The haploid oil palm plants may be used for producing homozygous doubled haploid oil palms: doubled haploids may be intercrossed to produce uniform F1 hybrids of superior properties.
