#$%^&*AU2020201707A120200326.pdf#####ABSTRACT The present disclosure provides a solid phase method of making oligonucleotides via sequential coupling cycles including at least one coupling of a dinucleotide dimer subunit to a free 3'-terminal group of a growing chain. The oligonucleotides include at least two nucleoside subunits joined by a N3'-*P5'phosphoramidate linkage. The method may include the steps of (a) deprotecting the protected 3' amino group of a terminal nucleoside attached to a solid phase support, said deprotecting forming a free 3' amino group； (b) contacting the free 3' amino group with a 3'-protected amino-dinucleotide-5'-phosphoramidite dimer in the presence of a nucleophilic catalyst to form an internucleoside N3'-*P5'phosphoramidite linkage； and (c) oxidizing (e.g., sulfurizing) the linkage. The compositions produced by the subject methods may include a reduced amount of one or more (N-x) oligonucleotide products. Also provided are pharmaceutical compositions including the subject oligonucleotide compositions. 149WO 2015/168310 PCT/US2015/028327 1/14 Components 8 0 .0 c% C0 04 ID I I N1 )C)0 M C14 -0C IC)c% C oc D <； 00L <； co r- CD 0 L 0N 00 C L * .N r 04 CD N LO 00 c) c) c) NN~ C) 0 ) 00co c% LOC ) Oci DL M M C) C C 'D o rF(00 C 1 0 0a0) co I-N sqD
