PURPOSE:To make it possible to carry out high-density culture of titled cell in a large amount, by bonding attaching animal normal cells to a fine granular biological high polymer carrier and embedding the cell in gel of calcium alginate. CONSTITUTION:Attaching animal normal cell are seeded in a microcarrier consisting of a biological high polymer such as gelatin, collagen, etc., in such a cell density that 5-10 cells are bonded to one carrier and cultivated in 5% carbonic acid gas concentration atmosphere for 1-3hr. to bond the cells to the carrier. The microcarrier is blended with 1.6% solution alginic acid obtained by dissolving alginic acid in 0.85% sodium chloride injection in such a way that the final concentration of alginic acid gel is 0.3-1.0%. The mixed solution is added from a nozzle or a sterilized pipette to a solution obtained by adding 0.001-0.01% Tween80 to sterile calcium chloride to give a cell embedded microcarrier, which is cultivated in a medium.
