Nozdrachev Aleksandr Danilovich (RU),Ноздрачёв Александр Данилович (RU),Goryukhina Olga Aleksandrovna (RU),Горюхина Ольга Александровна (RU),Martyushin Sergej Vasilevich (RU),Мартюшин Сергей Васильеви
申请号：
RU2019118349
公开号：
RU0002722822C1
申请日:
2019.06.13
申请国别(地区):
RU
年份:
2020
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to medicine, particularly to production of biodegradable nanospheres for inclusion of biologically active proteins in their composition for stabilization of their structure and directed delivery to target tissue with intranasal introduction. Method involves preparation of an aqueous solution of dextran and aqueous solution of protein, mixing them in an equal volume, adding to the obtained solution vegetable oil in amount of 3.5 volume of the volume of the initial solution, obtaining a mixture of two immiscible liquids, ultrasonic treatment and centrifugation of the mixture, drying the obtained nanosphere with diameter of not more than 200 nm. Protein used is a sublimation-dried total histone of animal origin, which is a complex of cationic proteins which is dissolved to 0.1 % concentration in distilled water. Nanospheres thus obtained are modified by a complex of core histones which are covalently bound to the surface of nanosphere, and nanospheres containing not less than 200 mcg of core histones per 1.0 g of nanosphere are obtained.EFFECT: invention provides preservation of native structure of protein due to its inclusion in composition of resorbable nanospheres intended for intranasal introduction, and modification of surface of nanospheres with positively charged proteins increases degree of absorption of nanospheres by olfactory mucous membrane.1 cl, 1 dwg, 3 exИзобретение относится к области медицины в частности к получению биодеградируемых наносфер для включения в их состав биологически активных белков для стабилизации их структуры и направленной доставки к ткани-мишени при интраназальном введении. Способ предусматривает приготовление водного раствора декстрана и водного раствора белка, их смешивание в равном объеме, добавление к полученному раствору растительного масла в количестве 3,5 объема от объема исходного раствора, получение смеси двух несмешивающихся жидкостей, ультразвуковую обработку и центрифугирование с
