COMPOSITIONS COMPRISING ADJUSTABLE CONCENTRATIONS OF GROWTH FACTORS DERIVED FROM BLOOD SERUM AND CLOT HYPOXIA-CONDITIONED MEDIUM AND METHODS OF THEIR PRODUCTION
Sarah Prinzessen Von Isenburg,Constance Neuhann-Lorenz
申请号：
US16339536
公开号：
US20190290690A1
申请日:
2017.10.04
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present invention is based on the idea of extracorporeally simulating a wound in order to obtain wound healing/regenerative growth factor mixtures. The invention provides a method for separating the growth factors that are released from cells upon coagulation (coagulation-induced signaling phase) from the growth factors that are secreted by cells under hypoxia (hypoxia-induced signaling phase), then mixing the two phases at defined ratios to obtain a novel composition. Peripheral blood is obtained and allowed to clot or centrifuged to induce clotting. The serum is removed completely or partially and replaced with fresh medium of volume equal or less than the volume of serum removed. The clot is incubated in fresh medium at a temperature of 10 to 40° C., under hypoxia (1-10% O2) for 1 to 7 days. The clot conditioned medium is then collected and mixed with the serum at a defined ratio to obtain the composition. Compositions comprising adjustable concentrations of growth factors derived from blood serum (coagulation-induced phase) and clot-conditioned medium (hypoxia-induced phase) can be added to suitable carriers, and can be topically applied or injected into a subject in order to aid wound healing, skin regeneration or rejuvenation.
