GARRY, Daniel J.,GARRY, Mary G.,RASMUSSEN, Tara,KOYANO-NAKAGAWA, Naoko
申请号：
EP16759528
公开号：
EP3264891A4
申请日:
2016.03.03
申请国别(地区):
EP
年份:
2018
代理人:
摘要：
Described herein is a method for producing a chimeric non-human animal expressing a human ETV2 gene comprising: a) generating an ETV2 null non-human animal cell, wherein both copies of the non-human ETV2 gene carry a mutation that prevents production of functional ETV2 protein in said non-human animal； b) creating an ETV2 null non-human blastocyst by somatic cell nuclear transfer comprising fusing a nucleus from said ETV2 null non-human animal cell of a) into an enucleated non-human oocyte and activating said oocyte to divide so as to form an ETV2 null non-human blastocyst； c) introducing human stem cells into the ETV2 null non-human blastocyst of b)； and d) implanting said blastocyst from c) into a pseudopregnant surrogate non-human animal to generate a chimeric non-human animal expressing human ETV2.
