The present invention relates to a system for cultivating, sterilizing, and cooling mushroom seeds using a heat pump. More specifically, the system comprises: a cultivating chamber which is equipped with a cultivating tank in which a seed for cultivating a mushroom is fed, and a warm water spraying means which sprays warm water into the cultivating tank, so that the seed for cultivating a mushroom is sterilized； a warm water tank in which medium temperature water is stored； a boiler which has a medium temperature water feeding end for feeding the medium temperature water stored in the warm water tank, a heating means for generating warm water by heating the fed medium temperature water, and a warm water discharging end for discharging the warm water to the warm water spraying means； a cool water tank in which cool water is stored； a cooling chamber which is equipped with a cooling tank in which the sterilized seed for cultivating a mushroom is fed, and a heat exchanger which is installed in the cooling tank and in which the cool water is fed so as to absorb the indoor heat of the cooling tank, so that the seed for cultivating a mushroom is cooled； and a heat pump which includes a compressor which compresses a fed refrigerant in order to discharge the same as a high temperature and high pressure refrigerant, a condenser for heating the medium temperature water which is heat-exchanged with the refrigerant discharged from the compressor so that the medium temperature water is fed to and discharged from the warm water tank through a separate line, an expanding valve which expands the refrigerant condensed in the condenser, and an evaporator for freezing the cool water which is heat-exchanged with the refrigerant expanded in the expanding valve so that the cool water is fed to and discharged from the cool water tank through a separate line.본 발명은 히트펌프를 이용한 버섯종균배양 살균 및 냉각 시스템에 대한 것이다. 보다 상세하게는, 버섯재배용 종균을 배양시키기 위한 시스템에 있어서, 내부에 버섯재배용 종균이 투입되는 배양탱크 및 상기 배양탱크 내부로 온수를 분무하는 온수분
