Коломійченко Олег Юрійович,Лукянчук Іван Іванович,Семенчук Леся Володимирівна,Варенніков Юрій Олександрович
申请号：
UA201314863
公开号：
UA91113U
申请日:
2013.12.18
申请国别(地区):
UA
年份:
2014
代理人:
摘要：
The method for production of entomogenous biopreparation “Komakhocyd” for protection of agricultural and decorative plants against pests comprises preparation of bio product, the active bacterial strain «Streptomyces avermitili» 51 is used, cultivation of producer strain is carried out on balanced nutrient medium containing saccharose, maize extract, magnesium sulfate, monosubstituted potassium phosphate, dry fermentative peptone, yeast autolysate, glycine, thiamine hydrochloride, pyridoxin hydrochloride, vitamin PP, and drinking water. The production of the target product is carried out by the following method: saccharose is diluted with distilled water till reaching of 30% amount in solution, and ingredients are introduced into the prepared solution. After that of nutrient medium is sterilized for 1 hour at the temperature of 130 °C, cooled to 25-30 °C. After that 10 ml of sterilized medium is placed into vial, the initial culture is placed into vial and cultivated for 48-72 hours at the temperature of 28-30°C . After that of nutrient medium is sterilized for 2 hours at the temperature of 130 °C, cooled to 25-32 °C. After that biomass titer is determined, placed into mini-fermenter having volume of . The medium is inoculated with 10 ml of biomass produced in the vial, and culture is grown in mini-fermenter for 48-72 hours at the temperature of 28-32 °C. Material sterility test is carried out, biomass titer and medium pH are determined. At that of produced biomass is placed into sterilized nutrient medium of the same composition, located in fermenter having volume of 1 tonne. The fermenter inoculation is carried out through the seeding union in flame zone, biomass is cultivated for 72 hours at the temperature of 25-30 °C at air supply by pressure up to 0.8 atm, and biomass is produced having the titer of 910. During the cultivation for control of bacterial growth in biomass the following samples are taken from apparatus: the first – after 2 hours after inoculation,
