<；P>；PROBLEM TO BE SOLVED: To provide maturation-induced dendritic cells for transplant, which are subjected to maturation induction ex vivo, are suspended in a medium and have little damage on cell-surface antigens. <；P>；SOLUTION: Immature dendritic cells are seeded on a surface of a substrate which is coated with 1.3-2.2 &mu；g/cm<；SP>；2<；/SP>；temperature-responsive polymer whose upper or lower critical solution temperature in water is 0-80°；C. After their maturation is induced using picibanil, the temperature of the culture liquid is regulated to a temperature not lower than the upper-limit critical solubilization temperature or not higher than the lower-limit critical solubilization temperature, and the matured dendritic cells are peeled off without subjecting them to any enzymatic treatment. <；P>；COPYRIGHT: (C)2009,JPO&INPIT
