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METHODS FOR PRODUCING AND USING MULTI-CHAPERON-ANTIGENIC COMPLEXES
专利权人:
ЭЙДЖЕНУС ИНК. (US)
发明人:
ЛЕКЛЕР Кеннет П. (US),ТОМЛИНСОН Эндрю Дж. (US)
申请号:
RU2011144575/10
公开号:
RU2011144575A
申请日:
2010.04.02
申请国别(地区):
RU
年份:
2013
代理人:
摘要:
1. A method for producing multichaperone-antigenic complexes, comprising: (a) contacting a biological sample with a solid phase with which HOP-affinity molecules are covalently coupled under conditions in which the multichaperon-antigenic complexes in a biological sample bind to said HOP-affinity molecules; (b) removal of unbound components in a biological sample from the solid phase; (c) elution of multichaperone-antigenic complexes from the solid phase; and (d) isolation of eluted multichaperone antigenic complexes. 2. The method of claim 1, wherein the sample is an extract of mammalian cells. The method of claim 2, wherein the sample is an extract of human cells. The method of claim 1, wherein the sample is an extract of tumor cells. The method of claim 1, wherein the sample is an extract of infected cells. The method of claim 1, wherein the sample is an extract of a genetically engineered cell. The method of claim 1, wherein said biological sample is a slip obtained by contacting an extract of tumor cells, an extract of cells infected with a pathogen, or an extract of cells transfected and expressing a nucleic acid encoding a tumor-associated antigen or a tumor-specific antigen or an antigen of an infectious disease, which contain cellular proteins, with a solid phase to which a member of a binding pair for a heat shock protein is bound. 8. The method of claim 7, wherein said solid phase to which said member of the binding pair is bound is an gp96-specific immunoaffinity column, and said heat shock protein is gp96.9. C�1. Способ получения мультишаперон-антигенных комплексов, предусматривающий:(a) приведение в контакт биологического образца с твердой фазой, с которой ковалентно связаны НОР-аффинные молекулы, в условиях, при которых мультишаперон-антигенные комплексы в биологическом образце связываются с указанными НОР-аффинными молекулами;(b) удаление несвязавшихся компонентов в биологическом образце с твердой фазы;(c) элюирование мультишаперон-антигенных комплек
来源网站:
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来源网址:
http://www.ckcest.cn/home/
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