The present disclosure provides hNGAL muteins that bind a pyoverdine family member or pyochelin and can be used in various application including pharmaceutical applications, for example, to inhibit or reduce growth of P. aeruginosa. The present disclosure also concerns methods of making one or more pyoverdine- or pyochelin-binding muteins described herein as well as compositions comprising one or more of such muteins. The present disclosure further relates to nucleic acid molecules encoding such muteins and to methods for generation of such muteins and nucleic acid molecules. In addition, the application discloses therapeutic and/or diagnostic uses of these muteins as well as compositions comprising one or more of such muteins.本案提供了hNGAL突變蛋白，其結合綠膿桿菌螢光素家族成員或綠膿桿菌螯鐵蛋白，並且可以用於各種應用，包括藥物應用，例如以抑制或降低綠膿桿菌的生長。本案亦關於生成本文中描述的一種或多種結合綠膿桿菌螢光素或綠膿桿菌螯鐵蛋白的突變蛋白的方法及包含一種或多種此類突變蛋白的組合物。本揭示內容進一步涉及編碼此類突變蛋白的核酸分子和用於生成此類突變蛋白和核酸分子的方法。另外，本申請揭示了這些突變蛋白及包含一種或多種此類突變蛋白的組合物的治療和/或診斷用途。
