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METHOD FOR PRODUCING RETINAL TISSUE
专利权人:
发明人:
申请号:
EP16844465.1
公开号:
EP3348631A1
申请日:
2016.09.08
申请国别(地区):
EP
年份:
2018
代理人:
摘要:
The present invention provides a production method of a retinal cell or retinal tissue, including the following steps:(1) a first step of culturing mammalian pluripotent stem cells in the absence of a feeder cell for a period not exceeding 30 days in a medium comprising 1) a factor for maintaining an undifferentiated state and 2) an MEK inhibitor,(2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.
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