The present invention provides a process for enhancing the germination of Horse-gram seeds thereby improving plant growth and vigour. The seed treatment process comprising optionally the treatment of Gamma radiation with a dose range of 100 Gy to 600 Gy and Ethyl Methane Sulphonate (EMS) in a range of 0.2 V/V to 0.6 V/V; and / or combination treatment of Gamma radiation and Ethyl Methane Sulphonate (EMS). The Seeds of Horse-gram mutant obtained by the present invention may be used for making ayurvedic preparations for controlling kidney stone, diabetes and weight lose. Further, it is useful for asthma, bronchitis, leucoderma, urinary discharge and heart diseases.