The present invention relates to a method for preparing a hone graft substitute using bovine bone, and more particularly to a method for preparing a sale hone graft substitute which does not have the risk of infection with bovine spongiform encephalopathy, the method comprising treating bovine hone with sodium hypochlorite and treating the treated bone at a high temperature of more than 600 (. The bone graft substitute does not cause an immune respon.se. because it is prepared by effectively removing lipids and organic substances from bovine bone having a structure very similar to that of the human bone. Also, it has excellent osteoconduetivity. and is free of prion, and thus it does not have the risk of infection with bovine spongiform encephalopathy. According to the disclosed invention, the bone graft substitute having such advantages can be prepared in a simple manner.
