This invention is to provide a pharmaceutical composition for selectively removing undifferentiated pluripotent stem cells, which produces cytotoxicity by increasing the concentration of potassium ions in undifferentiated pluripotent stem cells, so as to make undifferentiated pluripotent stem cells die, thereby obtaining specific cells cultured from pluripotent stem cells without being mixed with undifferentiated pluripotent stem cells.本發明係在提供一種選擇性去除未分化萬能幹細胞之醫藥組合物,藉由提高未分化之萬能幹細胞內鉀離子之濃度以產生細胞毒性,據以使未分化之萬能幹細胞死亡,從而使由萬能幹細胞培養所得之特定細胞,不夾雜有未分化之萬能幹細胞。