Yehoshua Shachar,Roger Kornberg,Ralph Eugene Davis
申请号：
US15597090
公开号：
US20180334697A1
申请日:
2017.05.16
申请国别(地区):
US
年份:
2018
代理人:
摘要：
A method of reducing the limit of detection in a surface acoustic wave sensor (SAW) includes the steps of: attaching a plurality of DNA segments to a detection surface of a SAW； performing a CRISPR/Cas9 preparation of the DNA segments to cut and splice a selected protein into at least one of a plurality of the DNA segments； conjugating a nanoparticle to the selected protein； and measuring the number of DNA segments with conjugated nanoparticles using a surface acoustic wave sensor (SAW). The nanoparticle may be modified to form a single electron transistor (SET) which generates a detectable signal in response to RF or ultrasonic excitation which is indicative of binding of the corresponding nanoparticle to a selected target analyte.
