A monovalent anti-canine parvovirus antibody is disclosed herein. The anti-canine parvovirus antibody as disclosed is scFv. The present invention further provides a recombinant vector and a host comprising the vector. The anti-canine parvovirus antibody as disclosed herein shows strong binding to canine parvovirus. The antibody is useful in development of composition for treatment or prevention against canine parvovirus.