PURPOSE: A method for manufacturing a fresh gastrodia fermentation extract is provided to manufacture the fermentation extract from the sap produced during the defrost drip of rapidly frozen fresh gastrodia through a biological reaction by fermentation microorganisms. CONSTITUTION: Gastrodia, which is the main ingredient, is washed and dried. The dried material is rapidly frozen for 4-30 hours maintaining the temperature at -40>；= to -70>；=. The sap is extracted by defrost drip by maintaining the temperature at 25>；= to 60>；= in a fermenter. The fresh gastrodia sap is mixed with fermentation microorganisms and distilled water in the fermenter. A mixture is produced by adding a nitrogen source and a carbon source needed for fermentation. The mixture undergoes a biological reaction in a bioreactor to produce liquefied lactic acid fermentation product. The lactic acid fermentation product is sterilized using a rapid sterilizer to sterilize the fermentation microorganisms. After impurities are removed using a diatomite filter, the lactic acid fermentation product is concentrated to have the sugar content of 25 to 60 Brix. The fermentation extract is dried with a spray dryer in order to have the moisture content of 5-7%. One nitrogen source is chosen among rice bran, barley flour, and wheat bran, and one carbon source among saccharose, maltose, and oligosaccharide. [Reference numerals] (AA) Gastrodia washing and drying； (BB) Rapid freezing； (CC) Thaw drip； (DD) Fermentation microorganism introduction； (EE) Bio-reaction(liquid fermentation)； (FF) Fermentation microorganism sterilization； (GG) Filtration； (HH) Concentration； (II) Spray-drying； (JJ) Raw material completion； (KK) Product application；본 발명은 생천마를 주원료로 하여 세척한 다음 건조하는 제1단계； 상기 제1단계의 건조물을 급속 동결한 다음, 발효조 내에서 해동드립하여 진액을 추출하는 제2단계； 상기 제2단계의 생천마 진액을 배양조 상에서 발효미생물과 증류수와 혼합한 다음, 발효에 필요한 질소원과 탄소원을 추가하여 혼합물을 생성하는 제3단계； 상기 제3단계의 혼합물을 생물반응기(bioreactor) 상에서 생물반응을 거쳐 액상화된 젖산발효산물을 생성하는 제4단계； 상기 제4단계의 젖산발효산물을 급속멸균장치(Out
