The present invention relates to a probe structure, a temperature measuring device having the probe structure, and a method of using the same. The probe structure comprises a light transmissive unit and one or more light sources. One or more light sources radiate one or more colors of light to and through the light transmissive unit. The temperature measuring device includes the above-mentioned probe structure and a temperature measuring body. The probe structure is assembled within the temperature measuring body. By the design of the light transmissive unit and the visual light provided by the light sources, the user can easily place the probe structure to the part to be measured.本發明係有關於一種探頭結構、具有該探頭結構之溫度量測裝置及溫度量測裝置之使用方法。探頭結構包括一透光頭及一或多數個發光源,且一或多數個發光源發出一或多數個顏色的光線,並發光以透出於透光頭。溫度量測裝置包括如上所述之探頭結構及一溫度量測本體,且探頭結構係組裝於溫度量測本體。溫度量測裝置之使用方法係利用發光源提供可見光並藉由透光頭之透光設計,可讓使用者容易地將探頭結構置放至待測部位。